 |
« Back to Search Results
23-3252-LesterWilcox-MUM
Necropsy pathology case report for IVAC Service ID 23-3252.
LAM-AI Case ID:
STUDY_23_3252
IVAC Service ID:
23-3252
Necropsy Date:
12/20/23
Species:
MUM
Principal Investigator:
Lester/Wilcox
Department:
Breeding Colony
Case Type:
necropsy pathology case
Case Focus:
veterinary diagnostic pathology
Reference Source:
IVAC pathology report
Case OverviewSEA-CDM entity:
study analysis
Final pathology diagnosis
SEA-CDM entity:
analysis
|
ANL_23_3252_FINAL_DIAGNOSIS
|
Reference: 23-3252
Histopathology confirms a retroorbital abscess from the Harderian gland, with abundant intralesional gram-negative coccobacilli. Bacterial culture of the lesion confirmed positive for Rodentibacter heylii, which is a common pathogen for conjunctivitis, otitis, rhinitis, and cervical lymphadenitis in mice and rats. Immunocompromised mice infected with Rodentibacter heylii also develop retrobulbar abscess of the lacrimal glands/Harderian glands, which may be associated with penetrating foreign bodies. In this case, no bacteria or other infectious etiology was found in other organs. Robust lymphoid proliferation was noted in thymus and some lymph nodes, resembling T cell lymphoma, which has been reported as a background finding in OT-1 mice (Laffey et al., 2022). However, the lymphoid proliferation didn't seem to impact the animal clinically in the present case. Ref: Laffey KG, Stiles RJ,
...
Ludescher MJ, et al. Early expression of mature αβ TCR in CD4-CD8- T cell progenitors enables MHC to drive development of T-ALL bearing NOTCH mutations. Proc Natl Acad Sci U S A. 2022 Jul 5;119(27):e2118529119. doi: 10.1073/pnas.2118529119. Epub 2022 Jun 29. PMID: 35767640; PMCID: PMC9271211.
Case Summary
Source Case Metadata
Parsed from
study.comments
Source workbook Master 2023 Necropsy Log.xlsx Source sheet 23-3252-LesterWilcox-MUM Necropsy Date 12/20/23 IVAC Service ID 23-3252 Species MUM Principal Investigator Lester/Wilcox Department Breeding Colony Case MetadataSEA-CDM entities:
experiment, group
necropsy pathology case
EXP_23_3252
|
Reference: 23-3252
|
IVAC pathology report
Case Record
Source Reference
Master 2023 Necropsy Log.xlsx::23-3252-LesterWilcox-MUM
Reference ID:
23-3252
Reference Source:
IVAC pathology report
Clinical History / Case Narrative
Nursing female mouse presented 12/13 with corneal ulceration and periorbital swelling of the left eye. Treatment with topical neo-poly-bac and flurbiprofen was initiated once a day. A recheck on 12/18 revealed significant swelling of the left side of the face, rendering the animal unable to open the eye fully. Lab reports that they have occasionally seen this in previous Rag2 KOs, but not for several months.
Group / Cohort Summary
Group ID
GRP_23_3252_01 Group key: grp_23_3252_01
Group Type
single animal
Group Size
1
Age Range
3 - 7 months
Composition
Species MUM Sex F Age 3-7 months (unclear from cage card) Genotype/Strain/Study Rag2-, OT-1+, Sf/f, Cre-
Group Notes
Single-animal pathology case.
SEA-CDM details
Animal / SubjectSEA-CDM entity:
subject
Tissues / SamplesSEA-CDM entity:
sample
Treatments / ProceduresSEA-CDM entities:
intervention, material
Pathology FindingsSEA-CDM entity:
occurrence
Diagnostic AssaysSEA-CDM entity:
assay
Results / ObservationsSEA-CDM entity:
results
36 result/observation record(s) are linked to this case.
Records are grouped by report/result section. Routine records with no findings are collapsed by default.
Final Diagnoses (2)
Unilateral harderian gland abscess with gram-negative coccobacilli
Final Diagnoses
pathology diagnosis
Process: Infectious
Organ/System: Special senses; Process: Infectious; Diagnosis: Unilateral harderian gland abscess with gram-negative coccobacilli
Subject: SUBJ_CLN00113835
Assay: ASSAY_23_3252_MICRO
Analysis: ANL_23_3252_FINAL_DIAGNOSIS
SEA-CDM details
Lymphoproliferation, thymus and lymph nodes
Final Diagnoses
pathology diagnosis
Process: Inherited/Genetic
Organ/System: Hematolymphoid; Process: Inherited/Genetic; Diagnosis: Lymphoproliferation, thymus and lymph nodes
Subject: SUBJ_CLN00113835
Assay: ASSAY_23_3252_MICRO
Analysis: ANL_23_3252_FINAL_DIAGNOSIS
SEA-CDM details
Microscopic Morphologic Diagnoses (2)
Abscess, locally extensive, severe, chronic active, with intralesional gram-negative coccobacilli, unilateral side of the skull
Microscopic Morphologic Diagnoses
pathology diagnosis
Process: Infectious
Organ/System: Harderian gland; Process: Infectious; Morphologic Diagnosis: Abscess, locally extensive, severe, chronic active, with intralesional gram-negative coccobacilli, unilateral side of the skull
Subject: SUBJ_CLN00113835
Sample: SAMP_23_3252_HARDERIAN_GLAND
Assay: ASSAY_23_3252_MICRO
Analysis: ANL_23_3252_FINAL_DIAGNOSIS
SEA-CDM details
Lymphoproliferation
Microscopic Morphologic Diagnoses
pathology diagnosis
Process: Inherited/Genetic
Organ/System: Thymus and multiple lymph nodes; Process: Inherited/Genetic; Morphologic Diagnosis: Lymphoproliferation
Subject: SUBJ_CLN00113835
Sample: SAMP_23_3252_THYMUS_AND_MULTIPLE_LYMPH_NODES
Assay: ASSAY_23_3252_MICRO
Analysis: ANL_23_3252_FINAL_DIAGNOSIS
SEA-CDM details
Microscopic Findings (17)
histopathology observation
Microscopic Findings
histopathology observation
Findings: Y
Organ/Slide: Mesenteric lymph nodes; Findings: Y; Description: In multiple nodes, the parenchyma is largely infiltrated by sheets of neoplastic lymphocytes, which have the same morphology found in the thymus.
Subject: SUBJ_CLN00113835
Sample: SAMP_23_3252_MESENTERIC_LYMPH_NODES
Assay: ASSAY_23_3252_MICRO
SEA-CDM details
histopathology observation
Microscopic Findings
histopathology observation
Findings: Y
Organ/Slide: Skull; Findings: Y; Description: On one side of the skull, the retroorbital area (Harderian gland and adjacent soft tissue) is replaced by a large abscess, consisting of lots of degenerate neutrophils mixed with colonies of gram-negative coccobacilli and cell debris, surrounded by fibrous connective tissue. This abscess compresses the skull, and there is a small portion of the bone being absent (osteolysis). The eye is displaced outward.
Subject: SUBJ_CLN00113835
Sample: SAMP_23_3252_SKULL
Assay: ASSAY_23_3252_MICRO
SEA-CDM details
histopathology observation
Microscopic Findings
histopathology observation
Findings: Y
Organ/Slide: Spleen; Findings: Y; Description: Decreased amount of lymphoid tissue (expected phenotype); extramedullary hematopoiesis
Subject: SUBJ_CLN00113835
Sample: SAMP_23_3252_SPLEEN
Assay: ASSAY_23_3252_MICRO
SEA-CDM details
histopathology observation
Microscopic Findings
histopathology observation
Findings: Y
Organ/Slide: Sternum; Findings: Y; Description: The bone marrow and a subcutaneous lymph node adjacent to it are infiltrated by large numbers of sheets of lymphocytes, with the same morphology found in the thymus.
Subject: SUBJ_CLN00113835
Sample: SAMP_23_3252_STERNUM
Assay: ASSAY_23_3252_MICRO
SEA-CDM details
histopathology observation
Microscopic Findings
histopathology observation
Findings: Y
Organ/Slide: Thymus; Findings: Y; Description: The structure of cortex and medulla is obliterated by sheets of neoplastic lymphocytes. These cells are medium in size, with scant cytoplasm, round nucleus, prominent multiple nucleoli, and coarse chromatin. Apoptosis is frequent. Mitoses are 5 per high power field.
Subject: SUBJ_CLN00113835
Sample: SAMP_23_3252_THYMUS
Assay: ASSAY_23_3252_MICRO
SEA-CDM details
Evaluated with no findings / routine observations (12)
histopathology observation
Microscopic Findings
histopathology observation
Findings: N
Organ/Slide: Adrenal Glands; Findings: N; No microscopic findings reported.
Subject: SUBJ_CLN00113835
Sample: SAMP_23_3252_ADRENAL_GLANDS
Assay: ASSAY_23_3252_MICRO
SEA-CDM details
histopathology observation
Microscopic Findings
histopathology observation
Findings: N
Organ/Slide: Bladder; Findings: N; No microscopic findings reported.
Subject: SUBJ_CLN00113835
Sample: SAMP_23_3252_BLADDER
Assay: ASSAY_23_3252_MICRO
SEA-CDM details
histopathology observation
Microscopic Findings
histopathology observation
Findings: N
Organ/Slide: Brain; Findings: N; No microscopic findings reported.
Subject: SUBJ_CLN00113835
Sample: SAMP_23_3252_BRAIN
Assay: ASSAY_23_3252_MICRO
SEA-CDM details
histopathology observation
Microscopic Findings
histopathology observation
Findings: N
Organ/Slide: Eyes and optic nerves; Findings: N; No microscopic findings reported.
Subject: SUBJ_CLN00113835
Sample: SAMP_23_3252_EYES_AND_OPTIC_NERVES
Assay: ASSAY_23_3252_MICRO
SEA-CDM details
histopathology observation
Microscopic Findings
histopathology observation
Findings: N
Organ/Slide: Heart; Findings: N; No microscopic findings reported.
Subject: SUBJ_CLN00113835
Sample: SAMP_23_3252_HEART
Assay: ASSAY_23_3252_MICRO
SEA-CDM details
histopathology observation
Microscopic Findings
histopathology observation
Findings: N
Organ/Slide: Kidneys; Findings: N; No microscopic findings reported.
Subject: SUBJ_CLN00113835
Sample: SAMP_23_3252_KIDNEYS
Assay: ASSAY_23_3252_MICRO
SEA-CDM details
histopathology observation
Microscopic Findings
histopathology observation
Findings: N
Organ/Slide: Liver; Findings: N; No microscopic findings reported.
Subject: SUBJ_CLN00113835
Sample: SAMP_23_3252_LIVER
Assay: ASSAY_23_3252_MICRO
SEA-CDM details
histopathology observation
Microscopic Findings
histopathology observation
Findings: N
Organ/Slide: Lungs; Findings: N; No microscopic findings reported.
Subject: SUBJ_CLN00113835
Sample: SAMP_23_3252_LUNGS
Assay: ASSAY_23_3252_MICRO
SEA-CDM details
histopathology observation
Microscopic Findings
histopathology observation
Findings: N
Organ/Slide: Mammary Glands; Findings: N; No microscopic findings reported.
Subject: SUBJ_CLN00113835
Sample: SAMP_23_3252_MAMMARY_GLANDS
Assay: ASSAY_23_3252_MICRO
SEA-CDM details
histopathology observation
Microscopic Findings
histopathology observation
Findings: N
Organ/Slide: Skeletal muscle; Findings: N; No microscopic findings reported.
Subject: SUBJ_CLN00113835
Sample: SAMP_23_3252_SKELETAL_MUSCLE
Assay: ASSAY_23_3252_MICRO
SEA-CDM details
histopathology observation
Microscopic Findings
histopathology observation
Findings: N
Organ/Slide: Stomach and intestines; Findings: N; No microscopic findings reported.
Subject: SUBJ_CLN00113835
Sample: SAMP_23_3252_STOMACH_AND_INTESTINES
Assay: ASSAY_23_3252_MICRO
SEA-CDM details
histopathology observation
Microscopic Findings
histopathology observation
Findings: N
Organ/Slide: Uterus and ovaries; Findings: N; No microscopic findings reported.
Subject: SUBJ_CLN00113835
Sample: SAMP_23_3252_UTERUS_AND_OVARIES
Assay: ASSAY_23_3252_MICRO
SEA-CDM details
Gross Lesions (12)
Hematolymphoid
Gross Lesions
gross pathology observation
Findings: Y
Evaluated: Y
Organ/System: Hematolymphoid
Area of congestion at cranial pole of spleen.
Subject: SUBJ_CLN00113835
Assay: ASSAY_23_3252_GROSS
SEA-CDM details
Hepatic
Gross Lesions
gross pathology observation
Findings: Y
Evaluated: Y
Organ/System: Hepatic
Liver is slightly pale.
Subject: SUBJ_CLN00113835
Assay: ASSAY_23_3252_GROSS
SEA-CDM details
Special senses
Gross Lesions
gross pathology observation
Findings: Y
Evaluated: Y
Organ/System: Special senses
Multilobulated, yellow, fluid-filled structure wrapping around dorsal and lateral aspects of the eye. Fluid inside is yellow with a creamy texture.
Subject: SUBJ_CLN00113835
Assay: ASSAY_23_3252_GROSS
SEA-CDM details
Evaluated with no findings / routine observations (9)
Cardiovascular
Gross Lesions
gross pathology observation
Findings: N
Evaluated: Y
Organ/System: Cardiovascular
No gross findings reported.
Subject: SUBJ_CLN00113835
Assay: ASSAY_23_3252_GROSS
SEA-CDM details
Endocrine
Gross Lesions
gross pathology observation
Findings: N
Evaluated: Y
Organ/System: Endocrine
No gross findings reported.
Subject: SUBJ_CLN00113835
Assay: ASSAY_23_3252_GROSS
SEA-CDM details
Gastrointestinal
Gross Lesions
gross pathology observation
Findings: N
Evaluated: Y
Organ/System: Gastrointestinal
No gross findings reported.
Subject: SUBJ_CLN00113835
Assay: ASSAY_23_3252_GROSS
SEA-CDM details
Integument
Gross Lesions
gross pathology observation
Findings: N
Evaluated: Y
Organ/System: Integument
No gross findings reported.
Subject: SUBJ_CLN00113835
Assay: ASSAY_23_3252_GROSS
SEA-CDM details
Musculoskeletal
Gross Lesions
gross pathology observation
Findings: N
Evaluated: Y
Organ/System: Musculoskeletal
No gross findings reported.
Subject: SUBJ_CLN00113835
Assay: ASSAY_23_3252_GROSS
SEA-CDM details
Nervous
Gross Lesions
gross pathology observation
Findings: N
Evaluated: Y
Organ/System: Nervous
No gross findings reported.
Subject: SUBJ_CLN00113835
Assay: ASSAY_23_3252_GROSS
SEA-CDM details
Reproductive
Gross Lesions
gross pathology observation
Findings: N
Evaluated: Y
Organ/System: Reproductive
No gross findings reported.
Subject: SUBJ_CLN00113835
Assay: ASSAY_23_3252_GROSS
SEA-CDM details
Respiratory
Gross Lesions
gross pathology observation
Findings: N
Evaluated: Y
Organ/System: Respiratory
No gross findings reported.
Subject: SUBJ_CLN00113835
Assay: ASSAY_23_3252_GROSS
SEA-CDM details
Urinary
Gross Lesions
gross pathology observation
Findings: N
Evaluated: Y
Organ/System: Urinary
No gross findings reported.
Subject: SUBJ_CLN00113835
Assay: ASSAY_23_3252_GROSS
SEA-CDM details
Additional Procedures (3)
Culture, periorbital tissue
Additional Procedures
culture result
Moderate Rodentibacter heylii
Subject: SUBJ_CLN00113835
Sample: SAMP_23_3252_PERIORBITAL_TISSUE
Assay: ASSAY_23_3252_CULTURE
SEA-CDM details
Tissues Frozen
Additional Procedures
sample handling procedure
Held back spleen, liver, and kidney.
Subject: SUBJ_CLN00113835
SEA-CDM details
Tissues Refrigerated
Additional Procedures
sample handling procedure
Swab of fluid from periorbital mass.
Subject: SUBJ_CLN00113835
SEA-CDM details
Analysis / Final DiagnosisSEA-CDM entity:
analysis
Final pathology diagnosis
ANL_23_3252_FINAL_DIAGNOSIS
| IVAC pathology report
| Reference: 23-3252
Input Data
clinical history
gross findings
microscopic findings
culture results
Interpretation / Final Diagnosis
Histopathology confirms a retroorbital abscess from the Harderian gland, with abundant intralesional gram-negative coccobacilli. Bacterial culture of the lesion confirmed positive for Rodentibacter heylii, which is a common pathogen for conjunctivitis, otitis, rhinitis, and cervical lymphadenitis in mice and rats. Immunocompromised mice infected with Rodentibacter heylii also develop retrobulbar abscess of the lacrimal glands/Harderian glands, which may be associated with penetrating foreign bodies. In this case, no bacteria or other infectious etiology was found in other organs. Robust lymphoid proliferation was noted in thymus and some lymph nodes, resembling T cell lymphoma, which has been reported as a background finding in OT-1 mice (Laffey et al., 2022). However, the lymphoid proliferation didn't seem to impact the animal clinically in the present case. Ref: Laffey KG, Stiles RJ, Ludescher MJ, et al. Early expression of mature αβ TCR in CD4-CD8- T cell progenitors enables MHC to drive development of T-ALL bearing NOTCH mutations. Proc Natl Acad Sci U S A. 2022 Jul 5;119(27):e2118529119. doi: 10.1073/pnas.2118529119. Epub 2022 Jun 29. PMID: 35767640; PMCID: PMC9271211.
Source
Master 2023 Necropsy Log.xlsx::23-3252-LesterWilcox-MUM
Linked SEA-CDM Records
Group:
GRP_23_3252_01
Document:
DOC_23_3252
SEA-CDM details
Source DocumentsSEA-CDM entity:
documentation
1
source document record(s) are linked to this case.
Document types: necropsy pathology report (1)
IVAC pathology report 23-3252
necropsy pathology report
DOC_23_3252
Source Reference
Master 2023 Necropsy Log.xlsx::23-3252-LesterWilcox-MUM
Source file reference only; direct public download is not available in VaxSEA.
IVAC pathology report:
23-3252
Source Study Key:
STUDY_23_3252
Creator / Role
Yao Lee, DVM, MS, PhD, DACVP
ID type: name
Role: Final Review Pathologist
Case Report MetadataCase Complete: Yes
Completion Date: 2024-01-17
Clinician: Lester/Martin
Prosector: Hasler
Gross Necropsy Pathologist: Lee
Other Contact: Michelle Drenth
SEA-CDM details
|